Subjects: Geosciences >> Marine Sciences submitted time 2023-06-05
Abstract： We used COI marker and mitochondrial control region (CoReg) marker to determine genetic structure and genetic diversity of Crown-of-thorns starfish (Acanthaster planci) (Asteroidea, Spinulosa) in the northwest South China Sea, the geographical population of A. planci among six locations: including Bombay Reef - LH, Discovery Reef - HG, Vuladdore Reef - YZ, Observation Bank - YY, Luhuitou Peninsula - LHT, Lincoin Island – DD, were preliminarily studied, and the causes are discussed. We amplified the DNA sequences of 81 individuals from six populations, 81 COI sequences with a length of 622bp and 81 CoReg sequences with a length of 583bp were obtained. The analysis of two mitochondrial markers showed that there is a diversity of differences within the geographic population (COI: h: 0.286-0.824, π: 0.00119-0.00272, k: 0.743-1.692; CoReg: h: 0.286-0.846, π: 0.00686-0.01457, k: 4-13.79), CoReg sequence fragments show higher diversity, geographic population DD shows the highest diversity, there is a certain genetic structure between geographic populations (COI: FST: -0.00394-0.40513; CoReg: FST: 0.11198-0.78571). The haplotype network diagram and STRUCTURE analysis show that the geographic populations of A. planci in the Northwest South China Sea may be divided into two genetic lineages, with the results of AMOVA analysis support a high level of genetic communication among the geographic populations of A. planci in the northwest South China Sea. Migration is used to predict a gene flow route from geographic population LH and YZ in the Xisha Islands to geographic population LHT through geographic population YY in the northwest direction. The surface currents in the South China Sea provids impetus for the inter-population dispersal of A. planci larvae, which further construct the gene flow model of A. planci in the northwest South China Sea.
Hits
YES
Hits
Downloads
Comment